Lithium Acetate with glycerol
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Jump to navigationJump to searchUsed to store synchronized chemically competent yeast cells. Note that here we use 30% glycerol, as it increases the transformation efficiency relative to the standard 15%.
Final concentration 1M LiAc
- Weigh 0.66g Lithium acetate
- Add 70ml H2O
- Mix till all is dissolved
- Add 30ml 100% Glycerol
- Set to pH4.9